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Donor-Donor Energy-Migration Measurements of Dimeric DsbC Labeled at Its N-Terminal Amines with Fluorescent Probes: A Study of Protein Unfolding
Xuejun Duan, Zhen Zhao, Jianping Ye, Huimin Ma,* Andong Xia,* Guoqiang Yang, Chih-Chen Wang*
The folding/unfolding of a protein structure has been studied through measuring the donor-donor energy migration between two fluorescent probes coupled selectively to the N termini of the homodimeric DsbC protein. The fluorometric strategy provides a convenient and reliable method to investigate conformational changes in dimeric proteins.
Angew. Chem. Int. Ed., 2004, 43, 4216-4219